Table I.
Gene name | Sense primers | Antisense primers |
---|---|---|
β-actin | 5′-CATCACTATCGGCAATGAGC-3′ | 5′-GACAGCACTGTGTTGGCATA-3′ |
HIF-1α | 5′-CAAAACACACAGCGAAGC-3′ | 5′-TCAACCCAGACATATCCACC-3′ |
HIF-1α-specific target sequence | 5′-AAAGAGGTGGATATGTCTGGG-3′ | 5′-TTTCTCCACCTATACAGACCC-3′ |
E-cadherin | 5′-ATTCTGATTCTGCTGCTCTTG-3′ | 5′-AGTCCTGGTCCTCTTCTCC-3′ |
ZO-1 | 5′-CAACATACAGTGACGCTTCACA-3′ | 5′-GACGTTTCCCCACTCTGAAAA-3' |
Vimentin | 5′-AATGACCGCTTCGCCAAC-3′ | 5′-CCGCATCTCCTCCTCGTAG-3′ |
Fibronectin | 5′-CCCCATTCCAGGACACTTCTG-3′ | 5′-GCCCACGGTAACAACCTCTT-3′ |
HIF-1α, hypoxia-inducible factor-1α; ZO-1, zonula occludens-1.