Table 1. Total TruSeq cDNA library yields made with a given amount of input total RNA.

Yields measured by Nanodrop of cDNA libraries resuspended in 25 µL of EB. The italicized samples were near the lower limit of detection, and when analyzed with a Bioanalyzer, showed abnormal size distribution of cDNA fragments.

Amount input RNA	Replicate A	Replicate B	Replicate C
40 ng	57 ng	425 ng	672 ng
50 ng	435 ng	768 ng	755 ng
60 ng	115 ng	663 ng	668 ng
70 ng	300 ng	593 ng	653 ng
80 ng	468 ng	550 ng	840 ng