Table 4.

Biological examination of pactamycin and synthetic analogs^a

Entry	Structure	Code number	A549 EC50 (lung cancer)	MDA-MB-231 EC50 (breast cancer)	SK-OV-3 EC50 (ovarian cancer)	MRC-5 EC50 (human lung fibroblast)
1		1	160 nM	124 nM	129 nM	53 nM
2		28	11.8 μM	10.4 μM	12 μM	n.t.b
3		ent-(1)	2.1 μM	1.2 μM	1.6 μM	933 nM
4		15a	800 nM	659 nM	1.4 μM	380 nM
5		15b	141 nM	556 nM	434 nM	314nM
6		15c	1μM	n.t.b	600 nM	582 nM
7		15d	777 nM	4μM	4 μM	682 nM
8		15e	884 nM	3.3 μM	1.6 μM	2326 nM
9		15f	324 nM	376 nM	145 nM	431 nM
10		15g	2.21 μM	1.84 μM	2.44 μM	860 nM
11		15h	760 nM	800 nM	436 nM	366 nM
12		21	6μM	n.t.b	3.8 μM	2933 nM
13		22a	n.t.b	n.t.b	n.t.b	n.t.b
14		22b	n.t.b	n.t.b	n.t.b	n.t.b
15		22c	n.t.b	n.t.b	n.t.b	n.t.b
16		26a	n.t.b	n.t.b	n.t.b	2063 nM
17		26b	n.t.b	n.t.b	n.t. b	10.9 μM
18		26c	32 nM	50 nM	7 nM	6.5 nM
19		29	83 nM	356 nM	91 nM	49 nM
20		27b	88 nM	203 nM	103 nM	129 nM
21		27c	114 nM	79 nM	80 nM	105 nM
22		27d	118 nM	300 nM	75 nM	100 nM
23		27e	194 nM	352 nM	436 nM	366 nM
24	R = Ac	27f	137 nM	458 nM	123 nM	132 nM
25		27h	175 nM	1.93 μM	86 nM	396 nM
26		27g	588 nM	2.44 μM	593 nM	778 nM

^aAssays were carried out as triplicates.

^bNot toxic.