Table 1. Modes of cell migration.

Trajectory	Characteristics		Condition	Trajectory	Characteristics		Condition
	Temporal	Dimensional			Temporal	Dimensional
2D Substrate				3D matrix
	Random	2D	Wild Type, Zyxin shRNA, Zyxin shRNA + Wild Type Zyxin, Talin shRNA, p130cas shRNA, FAK shRNA		Random	3D	Wild Type, VASP shRNA, crp1 shRNA, Zyxin VBDmu, Zyxin shRNA + Wild Type Zyxin
	Periodic	2D	Not Observed		Periodic	3D	Not Observed
	Random	1D	Not Observed		Random	2D	Not Observed
	Periodic	1D	Not Observed		Periodic	2D	Not Observed
	Unidirectional		Not Observed		Random	1D	α-actinin shRNA, Zyxin ΔABD
					Periodic	1D	Zyxin shRNA
1D Stripe					Unidirectional		p130cas shRNA
	Random	1D	Wild Type, α-actinin shRNA, Zyxin ΔABD
	Periodic	1D	Zyxin shRNA
	Unidirectional		Wild Type, Zyxin shRNA, α-actinin shRNA, Zyxin ΔABD

Left columns: schematics of possible and observed cell trajectories in 1D, 2D and 3D microenvironments. Middle columns: temporal and dimensional characteristics of cell migration. Right columns: types of cells (WT or shRNA-depleted of specific proteins), in which such migratory patterns were observed in this paper. On 2D substrates, focal adhesion proteins (zyxin, vinculin, talin, and so on) do not control the dimensional and temporal characters of migration of cells. However, in 3D matrices, zyxin controls both the dimensional and temporal characters of the cell trajectories. Moreover, the depletion of p130Cas and α-actinin, respectively, induces (mostly) 1D unidirectional and 1D random migration inside the 3D matrix. Finally, along the 1D stripes, depletion of zyxin and α-actinin induces periodic and random migration of cells, respectively. Note that the lines of a 1D random or periodic cell trajectory are in actuality and by definition identically overlapping, but are drawn slightly separated here for clarity.