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Primers of the third exon in the MYOC gene.
| Exon | Primer | Primer sequence (5′–3′) | Product size (bp) | Optimal annealing temperature (°C) |
|---|---|---|---|---|
| 3 | MYOC-3-1F | CTTCCGCATGATCATTGT | 352 | 58 |
| MYOC-3-1R | CTTCCGCATGATCATTGT | |||
| 3 | MYOC-3-2F | ATACTGCCTAGGCCACTGGAA | 440 | 58 |
| MYOC-3-2R | CCGCTATAAGTACAGCAGCATGAT | |||
| 3 | MYOC-3-3F | GCCTTCATCATCTGTGGCAC | 342 | 58 |
| MYOC-3-3R | CAGGCAGCTTTGACTGCTTT |
F, forward; R, reverse; MYOC, myocilin.
