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. 2015 Mar 6;35(5):1237–1245. doi: 10.3892/ijmm.2015.2128

Figure 4.

Figure 4

Geranium thunbergii (GT) enhances the transcriptional activity and nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) in RAW264.7 cells. (A) An Nrf2 reporter cell line was treated with sulforaphane (SFN; 5 μM) or the indicated amounts of GT for 16 h. Luciferase activity was normalized by the amount of total proteins. *p<0.05, **p<0.01 and ***p<0.001 compared to the control (first column). All data represent the means ± SD of 3 independent experiments. (B) The cells were treated with the indicated concentrations of GT for 8 h. SFN (5 μM) was used as a positive control. The amount of nuclear Nrf2 was estimated by western blot analysis. The amount of lamin B was used as an internal control. (C) The intensity of the bands was densitometrically quantified and calculated as the means ± SD of 3 independent experiments. *p<0.05, **p<0.01 and ***p<0.001 compared to the control (first column).