Figure 7.

(A) HEK293-FL reporter cells were incubated with the active molecules in the presence of Wnt3a-CM for 15 h, and luciferase activity was determined. (B) Cytosolic proteins were prepared from HEK293-FL reporter cells treated with the vehicle or the active meroterpenoids in the presence of Wnt3a-CM for 15 h and subjected to Western blotting. (C) Two CRT-positive colon cancer cell lines were incubated with different concentrations of the active meroterpenoids, and cell viability was determined by Cell-Titer-Glo assay. *p < 0.05 and **p < 0.01, compared with the vehicle control group.