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. 2015 Mar 18;35(5):1266–1272. doi: 10.3892/ijmm.2015.2145

Figure 2.

Figure 2

Nuclear factor (NF)-κB-inducing kinase (NIK) is a direct target of miR-518a-3p. (A) Schematic of the miR-518a-3p target sites in the NIK 3′ untranslated regions (3' UTRs). (B) Reporter-based miR-518a-3p target gene screening. A series of 3′ UTR-luciferase reporters was transfected into SW620 cells with or without miR-518a-3p-specific inhibitory RNA (anti-miR-518a-3p) or negative control RNA (NC). The relative values of the dual-luciferase assays are presented, and the data are presented as the means ± SD of 3 independent experiments. (C) Mutation-induced reporters. The red box represents the mutated target region. (D) miR-518a-3p negatively regulates the NIK 3' UTR, as analyzed by reporter assays (n=4, means ± SD). The data represent the means ± SEM, n=3 independent experiments. **P<0.01 vs. control.