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. 2015 Mar 18;35(5):1266–1272. doi: 10.3892/ijmm.2015.2145

Figure 4.

Figure 4

The loss of miR-518a-3p is responsible for constitutive nuclear factor (NF)-κB activation, abnormal cell growth and resistance to apoptosis in NCM460 cells. (A) The mRNA level of NIK was detected using RT-PCR in LoVo and NCM460 cells. (B) miR-518a-3p restoration by a retroviral vector inhibits NIK RNA accumulation in NCM460 cells. The quantification of NIK and mature miR-518a-3p expression is shown (n=3, means ± SD). (C) miR-518a-3p or NIK-specific shRNA (shNIK) expression downregulates NIK protein expression and inhibits the downstream non-canonical NF-κB pathway in NCM460 cells. (D) Increased nuclear translocation of RelA due to the expression of miR-518a-3p inhibitor in NCM460 cells. The data represent the means ± SEM, n=3 independent experiments. *P<0.05, **P<0.01 vs. control.