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. 2015 Apr;145(4):303–314. doi: 10.1085/jgp.201411292

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© 2015 Ohrtman et al.

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Figure 3. — IIF7, which has an epitope proximal to the truncation site, immunoprecipitates both sized species of Ca_V1.1, whereas the distal antibody only immunoprecipitates the larger species. Solubilized P1 muscle membranes were immunoprecipitated with either IIF7 (lanes 1 and 2) or with the distal antibody (lanes 3 and 4), or with the distal antibody preincubated with its generating peptide (lanes 5 and 6). Western blotting of the resulting pellets and supernatants with IIF7 demonstrates that the distal epitope is present only in the larger-sized form of Ca_V1.1. Note that lanes 3–6 are all from the same experiment, that lanes 3 and 5 were loaded identically, and that lanes 4 and 6 were loaded identically.