Fig. 6.
Molecular expression in male and female tail feathers. Longitudinal male and female tail feather sections (in regeneration) were stained for H&E, PCNA, Feather Keratin A (Ker A), Cytokeratin II (Cyto II) and β-catenin (Beta-Cat). The distributions of PCNA and β-catenin protein were determined using immunostaining. The distribution of Feather Keratin A and Cytokeratin II were determined using in situ hybridization. A high power view of the feather collar region is shown (H &E, PCNA, and Beta-Cat). Since there was no staining in the collar region for Ker A, the ramogenic zone is shown for Ker A and Cyto II with size bars = 250µm. A lower power view is shown in the upper insets with size bars = 0.5 mm and a higher power view is shown in the lower insets with size bars = 200µm.