Table 1.
PCR-validated gene targets of p300 HAT.The numbers indicate the relative expression level fold change in cells treated with C646 for 24 h over the DMSO control. Descriptions of gene functions are taken from PubMed gene search. “-” indicates that no broad cellular function has been associated with a particular gene.
Gene | Fold Change | Known Function(s) | |
---|---|---|---|
Repressed | |||
family with sequence similarity 111, member B | FAM111B | -42.5 | - |
histone cluster 1, H2bb | HIST1H2BB | -29.1 | chromatin assembly |
ubiquitin-like with PHD and ring finger domains 1 | UHRF1 | -14.5 | G1/S transition, p53-dependent DNA damage checkpoint |
cyclin E2 | CCNE2 | -12.7 | G1/S transition |
DEP domain containing 1 | DEPDC1 | -12.4 | Inhibition of apoptosis |
X-ray repair complementing defective repair in Chinese hamster cells 2 | XRCC2 | -11.3 | homologous recombination |
cyclin A2 | CCNA2 | -7.2 | G1/S and G2/M transition |
RAD51 homolog | RAD51 | -5.6 | homologous recombination |
Bloom syndrome, RecQ helicase-like | BLM | -5.3 | 3′-5′ helicase activity, suppression of inappropriate recombination |
breast cancer 2, early onset | BRCA2 | -4.2 | homologous recombination |
replication protein A2 | RPA2 | -2.6 | homologous recombination |
| |||
Overexpressed | |||
protease, serine, 35 | PRSS35 | 5.7 | - |
tripartite motif containing 38 | TRIM38 | 4.3 | - |
microRNA 34a | MIR34A | 2.6 | - |
tissue inhibitor of metalloproteinase 3 | TIMP3 | 2.4 | irreversible inactivation of metalloproteinases |
tumor protein p53 | TP53 | 1.2 | response to diverse cellular stresses, promotion of cell cycle arrest, apoptosis, senescence and DNA repair |