Fig. 4.

CKB affects the physiology of stably transfected Caco-2 cells. A: Cells were plated in 12-well plates and incubated at 37°C for 30 min. Cells were than trypsinized and counted. B: Cells were plated in collagen-coated Boyden chambers and incubated for 48 h. Filters were then stained with crystal violet and imaged by light microscopy (20×). C: Cells were suspended in 0.6% agar and incubated for 14 days at 37°C. Pictures were taken with a scanner (1 ×), and the average number of colonies per view is displayed in the bar graph. D: Caco-2 cells were plated on 10-cm plates and treated with 0.25 µg/ml doxurubicin for 1 h or left untreated. The cells were then incubated in normal medium for 2 weeks and photographed using a scanner (1 ×).