Fig. 5.

Cellular stress is modulated by CKB in Caco-2 cells. Stably transfected Caco-2 cells were plated in six-well plates. Medium was replaced with 10% FBS/DMEM containing the stated amounts of glucose, and the cells were incubated for 48 h at 37°C. Cells were then trypsinized and stained with trypan blue. Counting was done with a hemocytometer. The number of viable cells (A) and the percentage of viable cells (B) are shown. In (C) and (D), cells were incubated for 1, 2, or 4 h at 43°C and then moved to 37°C for 48 h. Cells were then trypsinized, stained, and counted as in (A) and (B).