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. Author manuscript; available in PMC: 2015 May 22.
Published in final edited form as: Cell Rep. 2014 May 10;7(4):1227–1238. doi: 10.1016/j.celrep.2014.04.006

Figure 4. APPL1 Mediates Insulin Signaling in an IRS1/2-Dependent Manner.

Figure 4

MEF cells derived from IRS1 KO mice (A), IRS2 KO mice (B), or 32D/IR and 32D/IR/IRS-1 cells (C) were infected by adenovirus overexpressing GFP or GFP plus APPL1. The infected cells were serum starved for 4 hr and treated with or without 100 nM insulin (5 min). Akt phosphorylation and protein expression levels were detected with specific antibodies as indicated.