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. 2015 Mar 31;10(3):e0122482. doi: 10.1371/journal.pone.0122482

Fig 3. Degranulation assay of derived MCs.

Fig 3

A. A representative result of flow cytometry on original CD34+ hematopoietic precursors using anti-FcεRI and anti-CD117 antibodies. B. A representative result of flow cytometry on derived mast cells using anti-FcεRI and anti-CD117 antibodies. C. Quantification analysis of FcεRI/CD117 double positive population in original CD34+ hematopoietic precursors and derived mast cells. D. Mast cell-released histamine in different group was assessed by ELISA assay. E. Mast cell-released MIF was assessed by ELISA assay, mean±SD, n = 3.