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. 2015 Mar 31;10(3):e0122554. doi: 10.1371/journal.pone.0122554

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© 2015 Bae et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) Ranking of candidate reference genes according to average expression stability (M value). The x-axis from left to right indicates the ranking of the genes according to their expression stability. (B) Determination of the optimal number of reference genes for normalization. The software calculates the normalization factor from at least two genes; the variable V defines the pairwise variation between two sequential normalization factors.