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. 2015 Mar 31;10(3):e0121752. doi: 10.1371/journal.pone.0121752

Fig 7. Quantitative measurement of the level of nuclear AR and PSA secretion in SQ40-treated LNCaP cells.

Fig 7

LNCaP cells were first cultured in growth media supplemented with 5% CSS for 48 hours and then treated with 3, 6 and 12 μg/mL of SQ40 in the presence or absence of 100 nM DHT for 72 hours. Nuclear fraction of AR obtained from the cell lysate and concentrations of PSA secreted into culture medium were measured using commercial available ELISA kit. (A) AR level was normalized to the total nuclear protein level while (B) the concentration of PSA were normalised to the total cell number. Data were expressed as means ± SEM of three independent experiments and indicated as percentage of 100 nM DHT-stimulated cells set at 100%. ** indicates p<0.01; ***, p<0.001 versus unstimulated control. ###, p<0.001 versus 100 nM DHT-stimulated cells.