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. 2015 Mar 31;10(3):e0120487. doi: 10.1371/journal.pone.0120487

Fig 1. Necrosis-like death induced by 4-HC in CCRF-CEM cells.

Fig 1

A. Three hundred thousand CCRF-CEM cells were seeded in 6-well plates and directly treated with 50 μM 4-HC. Cell death was evaluated after several time points (0, 3, 6, 12 and 24 hours) using 7-AAD and Annexin V-FITC staining. B. CCRF-CEM cells were seeded in 6-well plates and directly treated with the indicated doses of 4-HC, etoposide or vincristine in the presence or in the absence of 10 μM TAT-RasGAP317-326. After 24 hours of drug incubation, 7-AAD and Annexin V-FITC staining was performed to evaluate cell death. 4-HC, 4-hydroperoxycyclophosphamide.