Fig 6. Coronafacoyl phytotoxin production is greatly enhanced by overexpression of both cfaR and orf1.

S. scabies strains overexpressing cfaR alone, orf1 alone or cfaR + orf1 were cultured in SFMB medium for 7 days at 25°C, after which the culture supernatants were harvested and extracted with organic solvent. The resulting organic extracts were then analysed for CFA-L-Ile production by HPLC. Shown is the mean area of the CFA-L-Ile peak from six cultures of each strain, with error bars indicating the standard deviation. A S. scabies strain containing only the overexpression vector was used as a control in this experiment.