Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid	Description	Resistance †	Reference or source
Streptomyces scabies strains
87–22	Wild-type strain	n/a	[15]
ΔtxtA	S. scabies 87–22 containing a deletion of the txtA thaxtomin biosynthetic gene	ApraR	[29]
Escherichia coli strains
DH5α	General cloning host	n/a	Gibco-BRL
NEB 5- α	DH5 α derivative, high efficiency competent cells	n/a	New England Biolabs
BL21(DE3)	Protein expression strain	n/a	New England Biolabs
ET12567/pUZ8002	dam –, dcm –, hsdS –; nonmethylating conjugation host	KanR, CmlR	[30]
Plasmids
pET-30b	N- or C- terminal 6 × histidine fusion tag protein expression vector with T7 promoter and lac operator	KanR	Novagen
pET-30b/CfaR	pET-30b derivative carrying a DNA fragment for expression of the CfaRfull–HIS6 protein	KanR	This study
pET-30b/CfaR1.1	pET-30b derivative carrying a DNA fragment for expression of the CfaRΔLuxR–HIS6 protein	KanR	This study
pET-30b/CfaR1.3	pET-30b derivative carrying a DNA fragment for expression of the CfaRΔPAS–HIS6 protein	KanR	This study
pRLDB50-1a	Streptomyces expression plasmid; carries the strong, constitutive promoter ermEp*and integrates into theφC31 attB site	ApraR, ThioR	[19]
pRLDB51-1	scab79591 (cfaR) overexpression plasmid derived from pRLDB50-1a	ApraR, ThioR	[19]
pRLDB81	scab79581 (orf1) overexpression plasmid derived from pRLDB50-1a	ApraR, ThioR	This study
pRLDB891	cfaR + orf1 overexpression plasmid derived from pRLDB50-1a	ApraR, ThioR	This study

^† Apra^R, Thio^R, Kan^R and Cml^R = apramycin, thiostrepton, kanamycin and chloramphenicol resistance, respectively.

n/a = not applicable.