Table 1. Mutations found in THF bypass products and in the control plasmids.

Substrate	Nucleotide inserted opposite the THF	Additional mutations downstream of the THF position
		Type of mutation a	Distance from the lesion site (nt)	Position in the URA3 gene b	Position in the vector c
THF	C	A → G	- 444		4516
	A	C → T	- 378		4450
	A	G → C	- 368		4440
	A	C ins	- 367		4439
	A	TC → AA	- 24	629	4096
	C	T → A	+ 34	571	4038
	T	A → C	+ 46	559	4030
	A	A → C	+ 92	513	3984
	A	GAT del	+ 108	497	3968
	A	A → C	+ 168	437	3908
	C	C → T	+ 193	412	3883
	A	G → T	+ 213	392	3863
	A	G del	+ 338	267	3738
	T	G del	+ 341	264	3735
	T	A del	+ 498	107	3578
	C	G → C	+ 626		3450
	A	A del	+ 799		3277
	T	A del	+ 799		3277
	C	C → T	+ 980		3096
	C	G → C	+ 1064		3021
	A	A → T	+ 1397		2688
	T	C → T	+ 1405		2680
	A	C → T	+ 1529		2556
Control	NA	A del	- 498		4575
	NA	C → T	- 460		4537
	NA	C ins	- 368		4445
	NA	T del	- 325		4402
	NA	C → G	- 221	826	4298
	NA	C → T	- 34	639	4111
	NA	A → G	+ 236	369	3840
	NA	G del	+ 376	229	3700
	NA	A del	+ 498	107	3578
	NA	T → C	+ 610		3466
	NA	T del	+ 784		3292
	NA	C → T	+ 907		3178
	NA	C → T	+ 1012		3073
	NA	G → T	+ 1209		2876
	NA	C → T	+ 1242		2843
	NA	A → C	+ 1447		2638
	NA	C → T	+ 1621		2812

^aNucleotide changes in the strand complementary to the THF-containing strand are shown.

^bNumbering for the URA3 gene is from the first nucleotide of the open reading frame.

^cNumbering for the vector if from the first nucleotide following the ARS4 sequence.

nt, nucleotide; del, deletion; ins, insertion; NA, not applicable.