Table 3. Mutations found in UV-induced Ura+ revertants of the ura3-G764A strain and Canr controls.
Nucleotide change at positions 763–765 of the URA3 gene a | Additional mutations downstream of the presumed UV lesion | |||
---|---|---|---|---|
Mutation type a | Distance from the lesion site (nt) | Chromosomal position b | ||
Ura+ revertants(TLS at the site of ura3-G764A mutation) | GAC→GCC | A → T | - 635 | 116294 |
GAC→GTC | G → A | - 23 | 116906 | |
GAC→AAC | G → A | + 16 | 116946 | |
GAC→AAC | A → G | + 34 | 116964 | |
GAC→AAC | A → G | + 40 | 116970 | |
GAC→AAC | A ins | + 177 | 117107 | |
GAC→AAC | C ins | + 190 | 5i | |
GAC→GGC | A ins | + 262 | 77i | |
GAC→AAT | T ins | + 414 | 229i | |
GAC→GGC | A → T | + 631 | 446i | |
GAC→CAC | A ins | + 636 | 451i | |
GAC→AAC | C → T | + 772 | 587i | |
GAC→GTT | G → A | + 886 | 701i | |
GAC→AAC | G → A | + 968 | 783i | |
GAC→GTC | G → A | + 1654 | 1469i | |
GAC→AAC | G → T | + 2015 | 1830i | |
GAC→GCC | C → T | + 2155 | 1970i | |
Canr controls (no TLS at the site of ura3-G764A mutation) | NA | A → T | - 1774 | 115155 |
NA | T → C | - 181 | 116748 | |
NA | C → T | + 621 | 436i |
aNucleotide changes (bold) are shown for the coding DNA strand complementary to the strand containing the dipyrimidine sequence at positions 763–764.