Fig. 3.

Chk2 knockdown reduces HSV-1 replication in vitro. HCE cells harboring tetracycline-inducible expression of shRNA against Chk2 or nontargeting control were cultured in the presence of doxycycline (0.25 μg/ml) for 72 h to induce Chk2 knockdown. Following the induction, cells were infected with HSV-1 at MOI 0.1, and total DNA was collected at the indicated time points for analysis by qPCR with primers for the viral genome and GAPDH. Doxycycline was present in the medium for the entire duration of infection. Protein lysates were collected at the time of infection to verify knockdown by Western blot (inset). Nucleolin is a loading control. Values represent average Δ ΔC(t) ± SEM for a representative of two independent experiments. n = 2 reaction replicates.