Fig. 6.
Chk2 inhibition reduces HSV-1- associated apoptosis in explanted corneas. Ex vivo human corneas were infected with 1× 10 4 PFU/cornea and treated with Chk2 inhibitor II (10 μM) or mock treatment (DMSO). Corneas were flash-frozen at 48 h and processed for indirect immunofluorescence staining with antibodies against cleaved caspase-3. The counterstain is Hoechst 33258. A representative limbal field for each treatment is shown. n = 2 corneas per treatment.