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. 2015 Mar 31;10(3):e0118893. doi: 10.1371/journal.pone.0118893

Table 2. The Difference between MixtureTree Annotator and Other Active Visualization Tools.

Tool Availability Supported format Colorization Mutation occurred at given time Export Layouts Advantage Disadvantage
Dendroscope Free for academic use NeXML, Nexus or Newick format Assign color individually (Edit>Format) N/A .eps,. svg,. png,. jpg,. gif,. bmp,. pdf Rectangular, slanted, circular and radial views 1. Supports general functionalities for tree. algorithms, subtree collapse, coloring and re-rooting. 2. Shows tanglegram for two trees or networks. Not favorable for users to assign color based on a group of sequences
iTOL (interactive tree of life) Online version, free Newick, Nexus or PhyloXML No graphic interface of colorizing function for branches and nodes N/A .svg,. png,. eps,. pdf Rectangularand circular-ring views 1. Is flexible for users who need to construct a phylogeny based on well-known organisms with NCBI taxonomy IDs. 2. Clade could be collapsed by setting proportion of distance. View online only; Not favorable for users to assign the color of subtrees or a group of sequences
HyperTree Free for academic use PHYLIP, phyloXML, Newick Assign color individually by graphic inferface (Edit>Color) or color editing file N/A PHYLIP format Linear, radial, unrooted views 1. Contains simple interface and functionalities for users to draw and colorize a phylogeny 2. Users can re-root the phylogeny by selecting a given node Lack of important information in phylogenetics such as branch length, confidence value, node annotation
NJPlot Free for academic use Newick N/A N/A .pdf and. eps Rectangular Tree operations, including new outgroup assignment, rotation and subtree visualization, are useful for phylogenic display Limited functionality and not recommended for large datasets
HyperGeny Free for academic use, online and downloadable packages are both available Newick Can only assign one color for all nodes N/A Newick format Unrooted view 1. Can collapse the subtree and output the Newick file from chosen node. 2. Is available for large tree structures and datasets Only for hyperbolic visualization
CTree Free Newick Can assign color to selected subtree N/A .pdf, Newick format Rectangular and unrooted views 1. Has simplistic interface for user to generate a publishable tree along with distance, bootstrapping value, re-rooting and subtree colorization. 2. Comes with statistical analysis, such as calculation of subtype diversity ratio and subtype diversity variance distribution. 1. Lacks ability to handle large datasets. 2. Supports the input and output format only in Newick format and pdf file, respectively
Phylowidget Free, online and downloadable packages are both available Newick, NHX and Nexus Assigns color individually by graphic interface N/A .jpeg,. pdf,. png Rectangular, slanted, circular and unrooted trees 1. Has comprehensive visualization tool for reconstructing phylogenetic trees. 2. Can handle big datasets 1. Takes more time and memory to run the program. 2. Lacks intuitive color assignment for sequences
BAOBAB Free for academic use Newick, Nexus or XML format Can assign color for subtree based on attributes of sequence name N/A Newick, XML and Pag Rectangular and unrooted trees 1. Can add, delete or modify the node and/or tree structure. Is useful in generating an artificial tree to illustrate user ideas. 2. Color selection is useful for users who are familiar with color manager function. 1. Is not very intuitive in assigning the color of nodes and branches. 2. Has simple interface but lacks functionality, such as different tree layouts, topology algorithms
TreeIllustrator Free for academic use Newick, NEXUS N/A N/A .ps and. jpeg Phylogram, rectangular, radial and slanted cladograms 1. Has built-in tree of life browser and search engine. 2. Customizes phylogenetic trees and compares them with the current classification of organisms. Lacks annotating functionalities for sequence, such as branch length, nodes and confidence values
Archaeopteryx Free for academic use New Hampshire, Nexus, ToL response XML, or (deprecated) NHX format Color assigned in XML format N/A Newick, PHYLIP,. pdf,. gif,. jpeg,. png,. bmp Rectangular, unrooted, and circular trees 1. Displays features based on taxonomy. 2. Can show node events with size of subtree, number of taxonomies and duplications. Lacks graphic interface of color function for branches and nodes
MixtureTree Annotator Free for academic use Newick format Can easily assign the color based on the name of sequence in the color function Can annotate where and when the nucleotide substitution occurred in given sequence on the tree structure NEXUS, Newick format; graphic output including. eps,. pdf,. jpeg,. png,. emf,. gif,. raw,. bmp,. ps Rectangular, polar, radial trees 1. Easily incorporates colorization and mutation annotating function into FigTree phylogeny viewer. 2. Has intuitive interface for users to assign color to nodes and branches; automatically groups the sequences based on the names of sequences. 3. Has accessible view of every mutation event on tree layouts 1. Cannot collapse clade 2. Cannot integrate the taxonomy of organisms based on known classifications