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. 2010 Feb;298(2):C221–C236. doi: 10.1152/ajpcell.00323.2009

Fig. 1.

Fig. 1.

Filamin and vimentin coregulate cell spreading. A: confocal micrographs illustrate filamin A (left) and vimentin (center) recruited to the cell cortex during the earliest phases of cell spreading. Bar = 30 μm. B: high magnification confocal micrograph (bottom right, inset) illustrating the colocalization of filamin A and vimentin at the lamellipodium during cell spreading. Bar = 5 μm. C: confocal micrographs show presence of actin (top left), cortactin (top right), vinculin (bottom left), and paxillin (bottom right) in cell extensions. Cell extensions are evident in spreading cells (arrows). Bar = 30 μm. D: Western blots illustrating filamin A and/or vimentin knockdown by small interfering RNA (siRNA) in human embryonic kidney (HEK)-293 cells (left) and in mouse 3T3 cells (right). E: histogram showing differences in mean number of cell extensions ± SE among control, filamin-knockdown, vimentin-knockdown, and double-knockdown HEK-293 cells spreading on collagen. F: Alexa-488 phalloidin staining of control (far left), filamin-knockdown (left), vimentin-knockdown (right), and double-knockdown (far right) HEK-293 cells spreading on collagen. Bar = 30 μm. G: histogram showing differences in mean number of cell extensions ± SE between control, filamin-knockdown, vimentin-knockdown, and double-knockdown 3T3 cells spreading on collagen. H: Alexa-488 phalloidin staining of control (top left), filamin-knockdown (top right), vimentin-knockdown (bottom left), and double-knockdown (bottom right) 3T3 cells spreading on collagen. Bar = 30 μm.