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. 2010 Feb;298(2):C221–C236. doi: 10.1152/ajpcell.00323.2009

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Fig. 6. — Filamin binds vimentin in spreading cells and in vitro. A: coimmunoprecipitation and Western blotting show close association, in spreading cells, between filamin A and vimentin. B: dot-blot assay illustrating binding between purified FLAG-tagged filamin and GST-tagged vimentin. Fibronectin is used as a negative control. Dashed circle represents location of fibronectin application. C: filamin-vimentin binding is illustrated with pull-down assays using GST-tagged vimentin bound to glutathione beads (left) and FLAG-tagged filamin bound to anti-FLAG-coated beads (right).