Fig. 3.
Transfection of PTPα rescues FA turnover in PTPα−/− cells. A: transient transfection of PTPα−/− cells with hemagglutinin (HA)-tagged PTPα constructs. Cells were plated on fibronectin for 1 h and then stained with anti-paxillin antibodies. Transfected cells were identified by staining with anti-HA antibodies using a specific Texas Red (TR) secondary antibody (not illustrated). Arrows indicate supermature FA. D1/ΔD2, PTPα lacking D2 domain; ΔD1/D2, PTPα lacking D1 domain; ΔD1/ΔD2, PTPα lacking D1 and D2 domains. B: transient transfection of PTPα−/− cells with HA-PTPαwt. FA were visualized by cotransfection with GFP-α-actinin. Fibroblasts were allowed to spread for 1 and 3 h. FA turnover in rescued PTPα−/− cells is reflected by formation of smaller and more widely distributed FA. C: cell area after 3 h of spreading. Values are means ± SD of 50 measurements in 3 independent experiments. *P < 0.01 vs. control (by unpaired Student's t-test).