Fig. 6.

Effects of administration of everolimus in Nx rats. Nx rats were subcutaneously treated with vehicle (E−) or everolimus (E+, 2 mg/kg) for 14 days immediately after Nx. A and B: detection of mRNA of cyclin B2 (A) and Cdc2 (B) by real-time PCR. C: immunofluorescent analysis of Ki-67. D: numbers of stained nuclei for Ki-67 in the proximal tubules were counted in 3 independent regions at 100-fold magnification. E: appearance of remnant kidney. F: representative photographs of periodic acid Schiff (PAS) staining of the remnant kidney. G and H: measurement of glomerular diameter (G) and the height of epithelial cells (H). *, Glomeruli. Scale bars 100 μm. **P < 0.01, ***P < 0.001, significantly different from vehicle-treated (E−) rats.