Fig. 7.

Effects of administration of 2-cyanoethyl alsterpaullone (CE-ALP) in Nx rats. Nx rats were subcutaneously treated with vehicle (A−) or CE-ALP (A+, 0.5 mg/kg) for 14 days immediately after Nx. A and B: measurement of mRNA levels of Cyclin B2 (A) and Cdc2 (B) by real-time PCR. C: immunofluorescent analysis of Ki-67. D: numbers of stained nuclei in the proximal tubules were counted in 6 independent regions at 100-fold magnification. E: representative photographs of PAS staining of the remnant kidney. F and G: measurement of glomerular diameter (F) and height of epithelial cells (G). *, Glomeruli. Scale bars 100 μm. **P < 0.01, significantly different from vehicle-treated (A−) rats.