Fig. 8.

Effects of everolimus and CE-ALP on expression of phospho (p)-histone H3 and activities of Cdc2 in the kidney. A: representative photograph of kidney stained with p-histone H3 in sham-operated rats, Nx rats, and Nx rats treated with everolimus (E+) or CE-ALP (A+) at 2 wk after surgery. Dotted circles, glomeruli. Scale bars 100 μm. B: numbers of nuclei stained for p-histone H3 in the proximal tubules were counted at 100-fold magnification. C: activities of Cdc2 after administration of everolimus and CE-ALP. Multiple comparisons were performed with Bonferroni's test after a 1-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, significantly different.