Figure 3.
(a) Human jejunal enteroids plated on a fibronectin-based peptide-coated Transwell insert formed flat sheets of cells that merged to yield a monolayer with TER ~1000 Ωcm−2. Immunostaining with apical (villin), basolateral (Na+/K+-ATPase), and nuclear markers (Hoescht 33342) verified the polarized character of the single cell layer. (b) A similar differentiated monolayer of human duodenal enteroids expresses apical NHE3 and CFTR. (A color version of this figure is available in the online journal.)