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Human jejunal enteroids exposed to lentivirus stably express vector-encoded eGFP over a period of at least four weeks, suggesting transduction of Lgr5+ stem cells. Lentivirus transduction was carried out by spinoculation with mechanically dissociated enteroids. The panels show a single enteroid viewed by fluorescence (left panel) and bright field microscopy (right panel) obtained after four weekly passages. (A color version of this figure is available in the online journal.)
