. Author manuscript; available in PMC: 2015 Mar 31.

Published in final edited form as: Exp Biol Med (Maywood). 2014 Apr 9;239(9):1124–1134. doi: 10.1177/1535370214529398

Table 1.

Comparison of intestinal stem cell culture approaches

Characteristics	Crypt-derived enteroids^1–3	Tissue-derived organoids¹¹	iPSC/ESC-derived organoids¹⁰
Starting material	Biopsy or tissue	Murine neonatal or adult tissue	ES and iPS cells
Indefinitely expandable	Yes	No; proliferation potential decreases over time	No; limited to finite number of passages
Phenotype	Still under characterization	Neonatal/adult	More embryonic
Mesenchymal cells present	No	Yes	Yes
Production capabilities	Expand quickly	Dependent on tissue availability	Not dependent on tissue availability
Length of time to generate cultures	Less than 1 week	Less than 1 week	>1 month
Organization	Mimic crypt/villus unit	Mimic crypt/villus unit	Crypt/villus units with some random cell distribution