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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2015 Feb 12;149:118–127. doi: 10.1016/j.jsbmb.2015.02.004

Fig. 1. Up-regulation of c-MYC in AI resistant breast cancer cells.

Fig. 1

A. Real time PCR analysis of MYC expression in MCF7aro (hormone stripped for 72 hr) and LTEDaro cells treated with DMSO, testosterone (1nM) or testosterone (1nM) plus fulvestrant (100nM) for 6 hr. Values were normalized to ACTB and plotted relative to the expression of the MCF7aro cells treated with DMSO. Data are means ± SE (n=3).

B. Western blotting analysis of c-MYC expression in whole-cell lysates from MCF7aro or LTEDaro cells transfected with a nontargeting control (Ctrl) siRNA or a siRNA targeting c-MYC for 72 hr.

C. Cell proliferation of MCF7aro or LTEDaro cells transfected with a nontargeting control (Ctrl) siRNA or a siRNA targeting c-MYC. MTT was measured at the indicated time points. Data are means ± SE (n=5).