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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Virol Methods. 2015 Feb 16;217:1–7. doi: 10.1016/j.jviromet.2015.02.003

Figure 1.

Figure 1

Graphic representation of the MNV genome and description of the T7-IRES plasmid constructs used in this study. (A) Organization of the MNV genome showing the location of the virus nonstructural and structural protein genes. (B) Schematic diagrams of the T7-IRES clones carrying the MNV genome sequence: pT7CFE-MNV2T7 (FL MNV genome placed downstream of two T7pol promoters separated by the EMCV IRES sequence), pT7CFE-MNV5’ (FL MNV genome placed downstream of the T7pol promoter and the EMCV IRES sequence), pT7CFE-MNVΔ5’ (similar to the pT7CFE-MNV5’ construction with the MNV genome lacking the 5’-end NTR). Plasmids were engineered as described in text. First ATG codon of ORF1 is shown in bold. (C) Schematic diagram showing the site of the mKate2 gene insertion in the constructs: p20.3*-mKate, pT7CFE-MNV2T7-mKate, pT7CFE-MNVΔ5’-mKate and pT7CFE-MNV5’-mKate.