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Expression of the T7pol protein in the HEK293T-T7 cells. (A) Immunofluorescence staining of the HEK293T-T7 cells was performed using a mAb specific to T7pol (red). The nuclei were counterstained with DAPI (Sigma). (B) Active T7pol drove the expression of the GFP protein when the HEK293T-T7 cells were transfected with pT7CFEGFP as described in text. Original HEK293T cells were included as a negative control.
