Table 1.

PCR primers used in generation of FL MNV clones and site-directed mutagenesis of the MNV genome.

Name	Sequence 5’-3’
MNV sense pT7CFE2T7	5’ATATATATATCGTCTCTTTTGAAAAACACGATGATAATACGACTCACTATAGTGAAATGAGGATGGCAACGCC 3’
MNV sense pT7CFE5	5’ATATATATATCGTCTCTTTTGAAAAACACGATGATAATGTGAAATGAGGATGGCAACGCCATCTTCTGC 3’
MNV sense pT7CFEΔ5	5’ATATATATATCGTCTCTTTTGAAAAACACGATGATAATATGAGGATGGCAACGCCATCTTCTGC 3’
MNV reverse	5’ATATATATATCGTCTCACTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATGCATCTAACTACCAC 3’
Mutant SL1	5’ CGATGATAATGTGAAATGAGGATGGCAACACCTTCTTCTGCGCCCTCT GTGC 3’
Mutant SL2	5’ GGATGGCAACGCCATCTTCTGCACCAAGTGTGCGCAACACAG AGAAACGC 3’
Mutants SL1/2	5’ CGATGATAATGTGAAATGAGGATGGCAACACCTTCTTCT GCACCAAGTGTGCGCAACACAGAGAAACG

The viral sequences of the oligonucleotides MNV sense pT7CFE2T7, MNV sense pT7CFE5, MNV sense pT7CFED5 and MNV reverse are shown in bold. The T7pol promoter sequence is in italics and recognition sequence of the BsmBI restriction enzyme is underlined. For oligonucleotides Mutant SL1, Mutant SL2 and Mutant SL1/2 used for site-directed mutagenesis of the MNV genome, the introduced mutations are shown in bold and underlined.