The Jjj1 C-terminus is important for the role of Jjj1 in Arx1 recycling. A) Δjjj1 ARX1-GFP cells containing empty vector (—), or expressing Jjj1, Jjj11-339, Jjj11-550, Jjj11-389 or Jjj11-362 from the native promoter, were grown at 23°C prior to imaging by fluorescence and differential interference contrast (DIC) microscopy. Representative images show DIC, Arx1-GFP localization, localization of a nucleus-specific RFP fusion protein, RFP-Pus1, and an image overlay (merge). B) Wild-type, Δarx1, Δjjj1, and Δjjj1 Δarx1 cells containing empty vector, and Δjjj1 and Δjjj1Δarx1 cells expressing jjj11-339 from the native promoter [(jjj11-339) and (jjj11-339 Δarx1), respectively], were serially diluted, spotted on a minimal medium plate, and incubated at 23°C for 3 days. C) Δjjj1 cells expressing either Jjj1, no Jjj1 (-) or the indicated Jjj1 truncation from the native promoter were serially diluted, spotted on a minimal medium plate, and incubated at 23°C for 2.5 days.