Figure 2. Glucose uptake in mammary cancer cells is increased following short-term IL4 exposure.
4T1 parental cells were treated with vehicle or 10 ng/mL murine IL4 for 22 hours prior to a 2 hour exposure to 2-NBDG +/− 10 ng/mL IL4, and immediate analysis by flow cytometry. A) Flow cytometry trace of 2-NBDG uptake in 4T1 cells treated with IL4 (black) or vehicle control (grey). B) Quantification of Mean fluorescence intensity by flow cytometry representative of 2-NBDG uptake in 4T1 cells with IL4 or vehicle control.