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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Mar 4;1853(5):1219–1228. doi: 10.1016/j.bbamcr.2015.02.020

Figure 3. IL4Rα mediates enhanced expression of GLUT1 in mammary cancer cells.

Figure 3

4T1 sh-control clones were treated with vehicle or 20 ng/mL murine IL4 every other day for 8 days. A) Western blot analysis of deglycosylated glucose transporter 1 (GLUT1) upregulation in 4T1 sh-control clones over time. B) Quantification of GLUT1 mRNA expression normalized to murine ribosomal S18 in 4T1 sh-control clones 6 days after initial IL4 treatment. Quantification of IHC staining for GLUT1 in C) 4T1 sh-control (Ctl) and IL4Rα knockdown (KD) orthotopic mammary tumors (n = 8) and D) in metastatic lung tumors originating from sh-control (Ctl) or IL4Rα knockdown (IL4Ra KD) 4T1 cells in wild-type (WT) or IL4 knockout (IL4 KO) mice (n = 3–6). The upregulation of GLUT1 expression in response to IL4 was reproducible in three independent experiments, and IHC analysis of GLUT1 in 4T1 mammary tumors in two separate experiments.