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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Virol Methods. 2015 Feb 21;217:55–63. doi: 10.1016/j.jviromet.2015.02.014

Table 2.

HI and NI titers after different durations of RDE heat inactivation.

A(H7N2) antisera HI titersa NI titersb
1 h 2 h 4 h 8 h 1 h 2 h 4 h 8 h
NL/57 1280 761 1810 1280 5120 5120 5120 5120
NL/68 640 538 640 640 2560 2560 2560 2560
BI/68 640 453 640 640 20,480 20,480 20,480 20,480
BI/76 269 320 320 320 5120 3840 5120 5120
NL/82 640 538 640 640 160 320 226 226
NL/92 538 453 453 538 1280 1280 1280 1280
NL/09 160 160 160 160 2560 2560 2560 2560

A(H7N2) antisera were incubated with RDE for 16 h at 37 °C and heat inactivated at 56 °C for 1, 2, 4, or 8 h. The geometric mean titers were calculated from duplicate titers.

a

Recombinant A(H7N2) viruses were tested against their homologous anti-A(H7N2) ferret sera in the HI assay.

b

Recombinant A(H2N2) and wild type A(H3N2) viruses were tested in the NI ELLA against their N2-homologous A(H7N2) ferret sera.