Skip to main content
. Author manuscript; available in PMC: 2016 Apr 10.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Feb 28;459(3):430–436. doi: 10.1016/j.bbrc.2015.02.122

Figure 2. PKM2 is a novel member of the fetal gene program.

Figure 2

(A) 1) Tissue glycogen content as assessed by periodic acid-Schiff’s base staining (pictured) and by enzyme coupled spectrophotometric quantification (right graph). 2) Western blot of O-GlcNAcylated proteins in whole tissue lysates from vehicle and sunitinib treated hearts (B) Transcript analysis by qPCR demonstrating activation of the fetal gene program. (C) Analysis of PKM2 gene expression by 1) restriction enzyme (PstI) digest of the pyruvate kinase cDNA amplicon. Data is expressed as the percentage (%) of the PKM2 isoform (bands 2 and 3) Please see the data supplement for a more detailed explanation. 2) qPCR analysis of relative mRNA expression of each pyruvate kinase isoform. 3) Protein expression of each pyruvate kinase isoforms as assessed by western blot. *P < 0.05 and **P < 0.01.