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. Author manuscript; available in PMC: 2016 Apr 10.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Feb 28;459(3):430–436. doi: 10.1016/j.bbrc.2015.02.122

Figure 3. Activation of Hif1α promotes PKM2 induction in the heart and isolated adult mouse cardiomyocytes.

Figure 3

(A) 1) Western blot demonstrating increased nuclear localization of Hif1α in sunitinib treated hearts. 2) Relative expression of Hif1α and of genes known to be induced by Hif1α in the heart. (B) PstI restriction digest of PKM cDNA from wild type mice (n=3 per group) subjected to either normoxia (~21% O2) or 8% O2 for 24 hours. (C) Percent PKM2 expression in isolated adult cardiomyocytes treated with sunitinib, subjected to 1% O2 or treated with pharmacological mimetics of hypoxia, dimethyloxalylglycine (DMOG) or cobalt chloride for 24 hours. Data are ±SEM of 3–8 mice/wells per group. *P < 0.05.