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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Mar 6;1853(5):1229–1239. doi: 10.1016/j.bbamcr.2015.02.023

Figure 4. TGFβ1 enhances Snail and N-cadherin expression, and EMT in prostate cancer cells.

Figure 4

A. Representative images showing the effect of TGFβ1 (5 ng/ml for 3 days) on stress fiber formation (phalloidin staining) in PC3 and DU145 cells (x 40 magnification) and phase-contrast images showing the effect of TGFβ1 (5 ng/ml) on cell scattering in both cell lines (x 20 magnification). B. Bar graphs showing quantification of the cell scattering data in PC3 (left) and DU145 (right) cells (n= 4). C. Representative Western blot images of E-Cadherin, Keratin 8, N-cadherin, Vimentin, Snail, and Slug after one time treatment with TGFβ1 (5ng/ml) or no treatment for 12, 24, 48, and 72 h in PC3 and DU145 cells. D. Bar graph showing optical band densitometry analysis of N-cadherin expression in PC3 cells, after one time treatment with TGFβ1 (5ng/ml) or no treatment for 12, 24, 48, and 72 h normalized to β-actin (n= 4). E. Bar graph showing optical band densitometry analysis of N-cadherin expression in DU145 cells, after one time treatment with TGFβ1 (5ng/ml) or no treatment for 12, 24, 48, and 72 h normalized to β-actin (n= 4). F. Bar graph showing optical band densitometry analysis of Snail expression in PC3 cells, after treatments with TGFβ1 (5ng/ml) or no treatment for 12, 24, 48, and 72 h normalized to β-actin (n= 4). G. Bar graph showing optical band densitometry analysis of Snail expression in DU145 cells, after treatments with TGFβ1 (5ng/ml) or no treatment for 12, 24, 48, and 72 h normalized to β-actin (n= 4). *p<0.05 and **P<0.01, Data presented as Mean ± SEM.