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. Author manuscript; available in PMC: 2016 Apr 13.
Published in final edited form as: J Biomech. 2015 Feb 7;48(6):1058–1066. doi: 10.1016/j.jbiomech.2015.01.033

Table 1.

Molecular Beacon and Target Sequences

Sequence 5′ ⇨ 3′
Beacon
 PPARG 6FAM-ccggtcGCATGGAATAGGGGTTTGgaccgg-BHQ1
 Random (Quenchered) 6FAM-gaggcc AAAAAGTGTTGGAAATGTTGGAAAAAGggcctc-BHQ1
 Random (Quencherless) 6FAM-gaggcc AAAAAGTGTTGGAAATGTTGGAAAAAGggcctc
 GAPDH 6FAM-cgacgGAGTCCTTCCACGATACCAcgtcg-BHQ1
Target
 PPARG Target CAAACCCCTATTCCATGC
 Off-Target CTTATGAAATGGGCTTTAACA

Loop regions (reverse complement of target mRNA sequence) are capitalized. Stem regions are italicized lowercase. Where specified, 6-FAM fluorophores and BHQ1 quenchers were conjugated to 5′ and 3′ ends of sequences. DNA sequences were custom-designed with the exception of GAPDH (Nitin et al., 2004).