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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Methods. 2014 Dec 2;76:137–148. doi: 10.1016/j.ymeth.2014.11.012

Figure 6.

Figure 6

GUSSI plot of the gITC analysis in SEDPHAT of a simulated displacement experiment, titrating 300 µM of a high-affinity ligand (KAB = 1 nM) into 20 µM protein (purple), 355 µM of a low-affinity ligand (KAC = 1 µM) into 34 µM protein (blue), and the titration of 250 µM of the high-affinity ligand into a mixture of 20 µM protein with low-affinity ligand in 10fold molar excess (parameters after [81]). The inset shows the 1-dimensional error contour projection for the high-affinity binding constant as generated by an SEDPHAT statistics function, highlighting confidence levels at 1 (purple) and two (red) standard deviations, which leads to a 68% confidence interval of 0.57 – 1.4 nM.