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. Author manuscript; available in PMC: 2016 Mar 27.
Published in final edited form as: Circ Res. 2015 Feb 5;116(7):e40–e50. doi: 10.1161/CIRCRESAHA.116.304668

Figure 4. Immunohistology at the site of cell injection in MiPSC and c+AMC treated mice.

Figure 4

(A) MiPSCs showed successful engraftment by the detection of human mitochondrial antibody (green, white arrows) at the site of cell injection. (B) Immunostaining with cardiac troponin T antibody (green) did not demonstrate any signal within the engrafted MiPSCs (light blue) and labeled only the native murine myocardium. (C) c+AMCs showed successful engraftment by human mitochondrial antibody (green, white arrow), (D) Persistent ckit+ expression was confirmed by ckit+ receptor antibody (green, white arrow). (E–F) However, immunostaining using cardiac troponin T (green, white arrow) and PECAM antibodies did not co-localize with the human mitochondrial or ckit+ stain demonstrating no evidence of cardiac or endothelial differentiation, respectively. Nuclear counterstain (blue) and F-actin (red) antibodies were used to visualize the cellular cytoskeleton. (G) Merged and monochrome images of the MiPSCs co-stained with anti-luciferase antibody (FLUC), human nuclear antibody (HNA), and Hoechst 33342 (DNA). Robust co-localization of the 3 immunostains confirms the origin of the BLI signal from the transplanted human stem cells.