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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Cell Signal. 2015 Feb 26;27(6):1159–1167. doi: 10.1016/j.cellsig.2015.02.020

Figure 2. EHT 1864 inhibits GSIS in INS-1 832/13 cells.

Figure 2

INS-1 832/13 cells were cultured in RPMI media overnight in the presence of 2.5 mM glucose and 2.5% fetal bovine serum. After pre-incubation with EHT 1864 (0–10 μM) for 1 h, cells were incubated in the presence of low (2.5 mM) or high glucose (20 mM) in the continuous absence or presence of EHT 1864 for 30 min at 37°C. Insulin released into the medium was quantified by ELISA. The data was expressed as ng/ml of insulin released ± SEM. * p < 0.05 vs. 2.5 mM glucose and ** p < 0.05 vs. 20 mM glucose alone or in presence of 5 μM of EHT 1864.